Yashraj Biotech is seeking a highly motivated Scientist with 4–5 years of industrial experience in antibody bioconjugation, immunoassay development, and diagnostic reagent development. The candidate will work in the development and optimization of antibody-latex conjugates for immunoturbidimetric assays while collaborating with multidisciplinary R&D and Quality teams to deliver robust diagnostic products. Key Responsibilities • • Design, execute, and optimize antibody purification, characterization, and bioconjugation workflows. • Develop EDC/NHS-based covalent conjugation strategies for latex and nanoparticle-based diagnostic reagents. • Optimize antibody loading, blocking conditions, coupling chemistry, and conjugate stability. • Develop and optimize immunoturbidimetric assays (ITA) • Design and execute DOE-based optimization studies for conjugation efficiency and assay performance. • Evaluate analytical performance including sensitivity, specificity, linearity, precision, dynamic range, and robustness. • Troubleshoot conjugation, assay performance, and reagent stability issues. • Maintain accurate experimental documentation, SOPs, technical reports, and development records in compliance with GLP. • Mentor junior scientists, trainees, and interns in laboratory techniques and experimental planning. • Participate in technology transfer activities and support manufacturing scale-up of developed reagents. Cross-Functional Responsibilities • • Collaborate with Polyclonal and Monoclonal Antibody R&D teams for antibody selection and characterization. • Work closely with Quality Control (QC) during analytical validation and product performance evaluation. • Coordinate with Quality Assurance (QA) to ensure compliance with documentation, change control, and regulatory requirements. • Support Manufacturing in process optimization and troubleshooting during scale-up. • Participate in product development meetings, technical reviews, and root-cause investigations. • Contribute to continuous process improvement and new technology implementation. Required Technical Skills Antibody & Protein Sciences • • Strong understanding of monoclonal, polyclonal, artificial polyclonal antibodies. • Knowledge of antibody structure, affinity, avidity, stability, and antigen-antibody interaction kinetics. • Experience handling native and recombinant antigens and antibodies. • Hands-on experience in antibody purification using ammonium sulphate precipitation, dialysis, ultrafiltration, and related techniques. • Understanding of protein stability and conjugate characterization. Bioconjugation • • Hands-on industrial experience in EDC/NHS chemistry, Maleimide–Thiol Chemistry, Glutaraldehyde Coupling • Expertise in antibody immobilization onto latex beads , nanoparticles • Understanding of surface chemistry, functional group activation, antibody orientation, and conjugation efficiency • Experience in optimization of conjugation parameters for improved assay performance Latex/Nanoparticle Diagnostics • • Experience developing latex-based diagnostic reagents. • Understanding of particle size selection, surface modification, colloidal stability, blocking strategies, and storage stability. • Knowledge of reagent formulation and long-term stability assessment. Immunoassays • • Hands-on experience with Immunoturbidimetric Assays (ITA) • Working knowledge of ELISA and CLIA platforms • Experience in assay optimization, calibration curve generation, cut-off determination, and analytical validation Analytical Techniques Experience with: • • Immunoturbidometry • UV-Visible Spectrophotometry • Downstream processing of polyclonal antibodies • Dynamic Light Scattering (DLS) • Zeta potential analyser • Dialysis and ultrafiltration • Statistical analysis of assay performance Laboratory Practices • • Excellent laboratory skills including precision pipetting and sample handling. • Experience working under GLP/GMP environments. • Knowledge of laboratory safety, contamination control, and documentation practices. Desired Competencies • • Strong experimental design and scientific problem-solving skills. • Excellent troubleshooting and analytical thinking. • Ability to independently manage multiple R&D projects. • Strong written and verbal scientific communication. • Experience mentoring junior team members. • Good organizational and project management skills. • Ability to work effectively in cross-functional teams.