Perform complex Cell Biology, Molecular Biology, and Biochemistry techniques with minimal supervision including but not limited to: Mammalian cell culture Cell biological assays DNA/RNA isolation Molecular cloning qPCR Western blotting Protein extraction and purification Immunofluorescence microscopy Maintain mouse colonies which includes: Breeding and weaning Genotyping Harvesting tissues Performing physiological assessments Protocol Design and Refinements With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments. Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs). Record-keeping, Analysis, and Presentations Communicates effectively all aspects of experiments with PI or other laboratory staff. Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases. Catalogs specimens and samples. Performs independent analysis and interpretation of data for investigator . Operational Support Orders and catalogs laboratory supplies and supports laboratory operations. Operating, maintaining and troubleshoot highly specialized and sophisticated scientific apparatuses, equipment, and instruments. Knowledge, Skills, and Abilities: Aptitude for quick learning and multi-tasking. Knowledge of cell biology, molecular biology, and biochemistry principles and techniques. Expertise in cell culture, molecular cloning, nucleic acid analysis, protein biochemistry, and microscopy techniques. Skill in troubleshooting laboratory procedures and optimizing experimental protocols. Experience handling laboratory mice. Strong organizational, communication, and project management skills. Ability to work independently while contributing effectively within a collaborative research team.